Molecular Microscope® Diagnostic System (MMDx®) measures gene expression in biopsy samples of kidney and heart transplant patients. This whole-genome microarray chip technology uses machine-learning algorithm to calculate probability scores for particular rejection types, acute injury or fibrosis. MMDx is not intended to replace histology, but can be used for the objective assessment of challenging cases.
Reduce health costs associated with graft failure, dialysis, retransplantations and unnecessary rejection treatment.
Fill in the order form below. Then include the generated QR code in the shipment so we can recognize the sample.
Sampling tubes for standard biopsy.
Depending on severity of transplanted organ rejection or injury, particular genes are activated and produce unique patterns of RNA.
Using chip technology, thousands of different mRNA are quantified simultaneously, compared to reference sets of 5087 measured kidney and 3000 heart biopsies and evaluated by machine-learning algorithm.
The result is graphical output, where examined biopsy is displayed against the background of biopsy reference set which are coloured according to the type of rejection.
T-cell / B-cell mediated rejection
Mixed rejection
Acute injury
Inflammation
Irreversible atrophy-fibrosis
See how samples are handled after evaluation.
Fill in the order form below. Tubes will usually arrive within a week of your order being processed.
Fill in the order form below. The data from the form is not stored anywhere. After filling in the form, a QR code will be generated. Just print it and attach it to your shipment.
During standard biopsy of transplanted organ collect a minimum of 5 mm of kidney biopsy sample or at least 2 bites of endomyocard and immediately place it in the labeled sample tube with RNAlater®. The sample should be completely submerged in RNAlater®. Make sure that the sample tube screw cup is fully tightened. Place the labeled tube in the provided small clear biohazard bag, seal it and place it to FedEx Clinical Pak.
Ship samples to the Laboratory of Molecular Medicine IKEM (Centrální příjem vzorků IKEM, 4. patro, Vídeňská 1958/9,14021, Praha 4 Krč). All samples regardless of type of storage should be sent at room temperature.
a) Immediately after collection
b) For a maximum 6 days after collection: store at 4°C until shipping
c) Longer than 6 days after collection: leave sample at 4°C overnight to allow the
RNAlater® solution to penetrate the tissue. Move sample to -20°C.
Results are generally available within 1-2 (kidneys) or 3 (hearts) business days of receiving the sample. Some samples may require additional processing time if the RNA yield is low. The sample must be delivered to laboratory until 8 a.m. to start the analysis the day of delivery. In urgent cases, please, contact the lab to make arrangements using e-mail: mmdx@ikem.cz or mobile +420 607 040 579.
The results can be accesses by logging in to your account in the IKEM portal
under your login and password for 1 week. You will be notified by e.mail, when
the result is available. In case of any confusion regarding the MMDx ordering or interpretation of the result, please contact Laboratory of Molecular Medicine IKEM (mmdx@ikem.cz, tel: +420 607 040 579).
If you have any questions about MMDx testing, ordering or interpretation of the result, please contact the Molecular Medicine Laboratory of IKEM.
The result for kidneys is within 27 hours of the sample being delivered to the laboratory.
The result for the heart is within 51 hours of the sample being delivered to the laboratory.
If the sample does not arrive by 8am on a working day, the testing time will be extended by 24 hours.
Ústavy soudního lékařství
Kardiologie
Genetika
Pediatrická péče
1., 2., 3. LF UK a ÚVN Praha
IKEM
ÚBLG FN Motol – molekulárně genetická laboratoř a klinicko-genetické poradenství
Klinika Pediatrie FN KV Praha
FN Olomouc
Dětské kardiocentrum FN Motol
KEM (klinický genetik / kardiogenetik)
Ambulance pro rizikové děti a SIDS, Thomayerova nemocnice Praha
FN Hradec Králové
FN Olomouc
FN Olomouc (klinický genetik / kardiogenetik)
FN Ostrava
FN Ostrava – Oddělení klinické genetiky
Plzeň
FN Plzeň
Liberec
České Budějovice
The data from the form is not stored anywhere. After filling in the form, an order is generated. Just print it out and attach it to your shipment.
1. Order RNAlater™ Stabilization Solution (catalogue number AM7020, Thermo Fisher Scientific, 100 ml, which is sufficient for 100 samples).
2. Order 1.2 ml Nalgene cryogenic vials with external threads (catalogue V4757, bag of 500).
3. Prepare a box of cryogenic vials filled with 1 ml of RNAlater™ (pipetted with sterile pipette tips) and store at room temperature before use.
Please fill in the form to order, invoice will be issued to your e-mail address. Please note that the order will take time to ship to your location.
We are now processing the order. We will then confirm it to the email provided. Thank you, IKEM team
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